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Caspase 4 (Ich-2, ICErel-II, TX)

Caspase 4 (Ich-2, ICErel-II, TX)

Cat no: C2087-24A4

Supplier: United States Biological
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Caspase-4 (TX/ICH-2/ICE relII) is a member of the caspase family of proteases that play a key role in the execution of apoptosis and activation of inflammatory cytokines (1-3). Expression of caspase-4 has been observed in most tissues except brain, with highest levels in placenta, lung, spleen, and peripheral blood lymphocytes (PBL). Caspase-4 was originally found to contribute to Fas-mediated apoptosis (4). Several caspases (including caspase-4, caspase-5, and mouse caspase-11 and -12) are most closely related to caspase-1 and are capable of inducing apoptosis when over-expressed but are better characterized in the proteolytic activation of inflammatory cytokines (5). Caspase-4 associates with TRAF6 and is involved in the LPS inducible production of inflammatory cytokines IL-8 and MIP1 in THP-1 cells (6). While caspase-4 and mouse caspase-12 localize to the endoplasmic reticulum (ER) and may be activated by drugs that induce ER-stress (7), at least one study suggests that caspase-4 and caspase-12 are not essential for the ER-stress induced apoptosis (8). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2087-24A4
Reactivities: Human
Hosts: Rabbit
Applications: Western Blot
Size: 100ul
Form: Supplied in 10mM sodium HEPES pH 7.5, 150mM NaCl, 100ug/ml BSA and 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by Protein A affinity chromatography.
References: 1. Faucheu, C. et al. (1995) EMBO J 14, 1914-22, 2. Kamens, J. et al. (1995) J Biol Chem 270, 15250-6, 3. Munday, N.A. et al. (1995) J Biol Chem 270, 15870-6, 4. Kamada, S. et al. (1997) Oncogene 15, 285-90, 5. Martinon, F. and Tschopp, J. (2007) Cell Death Differ 14, 10-22, 6. Lakshmanan, U. and Porter, A.G. (2007) J Immunol 179, 8480-90, 7. Hitomi, J. et al. (2004) J Cell Biol 165, 347-56, 8. Obeng, E.A. and Boise, L.H. (2005) J Biol Chem 280.
Additional info: Recognizes human Caspase-4. Sequence Homology: monkey. Does not cross-react with other caspases.

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