Ready-to-use fluorometric substrate for caspase-6 and related caspases that recognize the amino acid sequence VEID. Caspase-6 and related caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate VEID-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized, using a hand-held long-UV lamp. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large amount of caspase-6 assays. Cell Lysis Buffer and 2X Reaction Buffer for caspase assays are also available separately.
Sequence: Ac-Val-Glu-Ile-Asp-AFC (AFC, 7-amino-4-trifluoromethyl coumarin)
Purity: (same/more than) 95% by HPLC analysis
Storage & Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -20 degrees C. Aliquots are stable for a minimum of 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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