Caspase 6 (McH2), Apoptosis Detection, Colorimetric BioAssay(TM) Kit

Cat no: C2087-25

Supplier: United States Biological

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Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Mch2/Caspase-6 Colorimetric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence VEID. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate VEID-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in Mch2/caspase-6 activity. Components: 25 assays 100 assays Cell Lysis Buffer 25ml 100ml 2X Reaction Buffer 2ml 4 x 2ml VEID-pNA Substrate (4 mM) 125ul 500ul DTT (1M) 100ul 400ul Dilution Buffer 25 ml 100 ml Stability: Store kit at -20 degrees C (Store Cell Lysis Buffer, Dilution Buffer and Reaction Buffer (2X) at 4 degrees C after opening). All reagents are stable for 1 year under proper storage conditions.

Catalogue number:	C2087-25
Size:	25Tests
References:	Product Specific: Doostzadeh J., et al. (2000) J. Biol. Chem. 275:25336-25341. General: 1. Martin, L.M., et al. , J. Biol.Chem. 272:7421-7430 (1997). 2. Thornberry, N.A., et al., J. Biol.Chem 272:17907-17911 (1997).

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Caspase 6 (McH2), Apoptosis Detection, Colorimetric BioAssay(TM) Kit

Cat no: C2087-25

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