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Caspase 8 Apoptosis Detection, Colorimetric BioAssay(TM) Kit (FLICE, MASH, Mch5)

Caspase 8 Apoptosis Detection, Colorimetric BioAssay(TM) Kit (FLICE, MASH, Mch5)

Cat no: C2087-40

Supplier: United States Biological
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Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase 8 Apoptosis Detection, Colorimetric BioAssay(TM) Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate IETD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in FLICE activity. Kit Components: 25 assays 100 assays 200 assays C2087-40A: 2X Reaction Buffer 2ml 4x2ml 16ml C2087-40B: IETD-pNA (4mM) 125ul 500ul 2x500ul C2087-40C: DTT (1M) 100ul 400ul 400ul C2087-40D: Cell Lysis Buffer 25ml 100ml 100ml C2087-40E: Dilution Buffer 25ml 100ml 200ml Storage and Stability: Store kit at -20 degrees C before use. After opening, store Cell Lysis Buffer (C2087-40D), 2X Reaction Buffer (C2087-40A) and Dilution Buffer (C2087-40E:) at 4 degrees C. Note: Protect IETD-pNA (C2087-40B) from light

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