Caspase 8 Apoptosis Detection, Fluorometric BioAssay(TM) Kit (FLICE, MASH, Mch5)

Cat no: C2087-30

Supplier: United States Biological

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Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. USBio's Caspase-8 Apoptosis Detection Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on detection of cleavage of substrate IETD-AFC (7-amino-4-trifluoromethyl coumarin). IETD-AFC emits blue light. Upon proteolytic cleavage of the substrate by caspases, free AFC emits a yellow-green fluorescence. The increase in the yellow-green fluorescence is proportional to the increase in caspase activity. Kit Contents: Assays 25 100 200 400 Cell Lysis Buffer 25ml 100ml 100ml 100ml 2X Reaction Buffer 2ml 4x2ml 16ml 32ml IETD-AFC (1mM) 0.125ml 0.5ml 2x.05ml 2x1ml DT (1M) 0.1ml 0.4ml 0.4ml 0.4ml Storage and Stability: Store kit at -20 degrees C before use. After opening, store Cell Lysis Buffer and 2X Reaction Buffer at 4 degrees C. All reagents are stable for 6 months under proper storage conditions.

Catalogue number:	C2087-30
Size:	25Assays
References:	Product Specific: 1. Brian S., et al. (2002) Pharmacol & Exp Therapeut. 302:8-17. 2. Coletti, D., et al. (2002) EMBO J. 21:631-642. General: 1. Nicholson, D.W., et al., Nature, 376:37-40 (1995) 2. Datta, R., et al., J. Biol. Chem. 272:1965-1969 (1997) 3. Thornberry, N.A., et al., J. Biol.Chem. 272:17907-17911 (1997)

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Caspase 8 Apoptosis Detection, Fluorometric BioAssay(TM) Kit (FLICE, MASH, Mch5)

Cat no: C2087-30

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