Caspase 8 Colorimetric Substrate, N-acetyl-Asp-Glu-Val-Asp-pNA p-nitroanilide (FLICE, MASH, Mch5)

Colorimetric substrate for Caspase-8. Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. See Caspase-8 Apoptosis Detection Kit C2087-40 which provides a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on detection of cleavage of substrate IETD-pNA (p-nitroanilide).\n\nUpon proteolytic cleavage of the substrate by caspases, free pNA is detected at a wavelength of 400nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the increase in Caspase-3 activity.\n\nFLICE and related caspase activity can be quantified by spectrophotometric detection of free pNA (lmax 400nm) after cleavage from the peptide substrate IETD-pNA, using a spectrophotometer or multi-well plate reader.\n\nPeptide sequence: Ac-Ile-Glu-Thr-Asp-pNA

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SPECIFICATIONS

Catalog Number

C2087-41

Size

200Tests

Form

Supplied as a liquid in DMSO (4mM).

Purity

(same/more than) 95% (HPLC)

References

Collin C., et al. (2001) J. Biol. Chem. 276:37602-37611.\nDoostzadeh J., et al. (2000) J. Biol. Chem. 275:25336-25341.\nGomez-Angelats M., et al. (2000) J. Biol. Chem. 275:19609-19619.\nSawa A., et al. (1999) Nature Medicine 5:1194-1198.\nShain, K.H., et al. (2002) J. Immunol. 168:2544-2553.\nVaradhachary, A.S., et al. (2001) J. Immunol. 166: 6564-6569.\nGeneral References:\nDatta, R., et al., J. Biol. Chem. 272:1965-1969 (1997)\nNicholson, D.W., et al., Nature, 376:37-40 (1995)