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Caspase 9, Colorimetric Substrate, LEHD-pNA (ICE-LAP6, Mch6, Apaf 3)

Caspase 9, Colorimetric Substrate, LEHD-pNA (ICE-LAP6, Mch6, Apaf 3)

Cat no: C2088-37

Supplier: United States Biological
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Ready-to-use colorimetric substrate for caspase-9/Mch6 and related caspases that recognize the amino acid sequence LEHD. Caspase-9 and related caspase activity can be quantified by spectrophotometric detection of free pNA (l = 400 nm) after cleavage from the peptide substrate LEHD-pNA, using a spectrophotometer or multi-well plate reader. Sequence: Ac-Leu-Glu-His-Asp-pNA Purity: (same/more than) 98% by HPLC analysis Storage & Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -20 degrees C. Aliquots are stable for a minimum of 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2088-37
Size: 200Tests
References: Product Specific: Chauhan D., et al. (2001) J. Biol. Chem. 276:24453-24456. Collin C., et al. (2001) J. Biol. Chem. 276:37602-37611. Datta R., et al. (2000) J. Biol. Chem. 275:31733-31738. Doostzadeh J., et al. (2000) J. Biol. Chem. 275:25336-25341. Matassa, A.A., et al. (2001) J. Biol. Chem. 276:29719-29728. Sawa A., et al. (1999) Nature Medicine 5:1194-1198.

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