Apoptosis, a morphologically distinguished form of programmed cell death, is critical during development and tissue homeostasis and plays a role in the pathogenesis of a variety of diseases. The morphological features of apoptosis and demise of the cell are because of the cleavage of structural and nonstructural proteins by a family of proteases called caspases. Upon stimulation by apoptotic stimuli, proximal caspases are activated and cleave downstream effector caspases leading to an amplification of proteolytic activity within the dying cells. Caspase-a and -b, previously called Caspy and Caspy2, were two zebrafish caspases containing N-terminal pyrin domains. Biochemical analysis revealed that both Caspy and Caspy2 are active caspases, but they exhibit different substrate specificity. Identification and initial characterization of the zebrafish orthologue of ASC (zAsc) and two zebrafish pyrin-containing caspases, Caspy and Caspy2 was also reported.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 1:5000-1:20,000
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.
Control Peptide: C2086-03D
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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