Ready-to-use colorimetric substrates for assaying activities of members of caspase family proteases. These substrates are formulated in a kit in the ready-to-use format. YVAD is the recognition sequence for caspase-1/ICE, VDVAD is the recognition sequence for caspase-2/ICH-1, DEVD is the recognition sequence for caspase-3/CPP32, WEHD is the recognition sequence for Caspase-5/ICErelIII, VEID is the recognition sequence for caspase-6/Mch2, IETD is the recognition sequence for caspase-8/FLICE, and LEHD is the recognition sequence for Caspase-9/Mch6.
Kit Components:
C2086-10B1: Caspase-1 Substrate, Ac-YVAD-pNA, 1 x 125ul
C2086-10B2: Caspase-2 Substrate, Ac-VDVAD-pNA, 1 x 125ul
C2086-10B3: Caspase-3 Substrate, Ac-DEVD-pNA, 1 x 125ul
C2086-10B4: Caspase-5 Substrate, Ac-WEHD-pNA, 1 x 125ul
C2086-10B5: Caspase-6 Substrate, Ac-VEID-pNA, 1 x 125ul
C2086-10B6: Caspase-8 Substrate, Ac-IETD-pNA, 1 x 125ul
C2086-10B7: Caspase-9 Substrate, Ac-LEHD-pNA, 1 x 125ul
C2086-10B8: Cell Lysis Buffer, 1 x 100ml
C2086-10B9: Dilution Buffer, 1 x 160ml
C2086-10B10: 2X Reaction Buffer, 1 x 20ml
C2086-10B11: DTT (1M), 1x400ul
Substrate Concentration:
4mM, supplied as a liquid in DMSO.
Storage & Stability:
Store all components at -20 degrees C. Stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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