Fluorometric substrates for assaying activities of members of caspase family proteases. These substrates are formulated in a kit in the ready-to-use format. YVAD is the recognition sequence for caspase-1/ICE, VDVAD is the recognition sequence for caspase-2/ICH-1, DEVD is the recognition sequence for caspase-3/CPP32, WEHD is the recognition sequence for Caspase-5/ICErelIII, VEID is the recognition sequence for caspase-6/Mch2, IETD is the recognition sequence for caspase-8/FLICE, and LEHD is the recognition sequence for Caspase-9/Mch6.
Kit Components:
C2086-10E1: Caspase-1 Substrate, Ac-YVAD-AFC, 1x125ul
C2086-10E2: Caspase-2 Substrate, Ac-VDVAD-AFC, 1x125ul
C2086-10E3: Caspase-3 Substrate, Ac-DEVD-AFC, 1x125ul
C2086-10E4: Caspase-5 Substrate, Ac-WEHD-AFC, 1x125ul
C2086-10E5: Caspase-6 Substrate, Ac-VEID-AFC, 1x125ul
C2086-10E6: Caspase-8 Substrate, Ac-IETD-AFC, 1x125ul
C2086-10E7: Caspase-9 Substrate, Ac-LEHD-AFC, 1x125ul
C2086-10E8: Cell Lysis Buffer, 1x100ml
C2086-10E9: Reaction Buffer, 2X 1x20ml
C2086-10E10: DTT, 1M, 1x400ul
Substrates are supplied as a 1mM liquid in DMSO.
Substrate Purity: (same/more than)95% by HPLC analysis
Storage & Stability:
For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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