Caspase Family Group III, Active, Recombinant (Caspases-6,8,9,10)

Caspase family proteases play an important role in apoptosis. Currently, the family consists of more than 10 members. Based on their extended substrate specificities, these enzymes were divided into three major groups: \nGroup I (contains caspase-1,-4,-5)\nGroup II (contains caspase-2,-3,-7)\nGroup III (contains caspase-6,-8,-9,-10). \n\nEvidences have suggested that Group I caspases are involved primarily in the production of inflammatory cytokines, while group II and III enzymes function in apoptosis as effectors and upstream activators, respectively. Active recombinant human caspases were expressed in E. coli and routinely tested for their ability to enzymatically cleave the substrate VEID-pNA (for caspase-6), IETD-pNA (for caspase-8 & -10) and LEHD-pNA (for caspase-9), respectively. \n\nHighly active recombinant human caspases were expressed in E. coli and purified. Active recombinant caspases are useful in screening caspase inhibitors, studying enzyme kinetics and regulation, determining target substrates and as a positive control in caspase activity assays. We recommend using 1 unit per assay for analyzing caspase activity.\n\nKit Components:\nC2086-10L1 (C2087-28A): Active Recombinant Caspase-6 1x25 units\nC2086-10L2 (C2087-60A): Active Recombinant Caspase-8 1x25 units\nC2086-10L3 (C2088): Active Recombinant Caspase-9 1x25 units\nC2086-10L4 (C2088-42): Active Recombinant Caspase-10 1x25 units\n\nUnit Definition: \nOne unit of the active recombinant caspase-6,-8,-9 & -10 is the enzyme activity that cleaves 1nmol of the caspase substrate VEID-pNA (for caspase-6), IETD-pNA (for caspase-8 & -10), and LEHD-pNA (for caspase-9), respectively, per hour at 37C in a reaction solution containing 50mM Hepes, pH 7.2, 50mM sodium chloride, 0.1% Chaps, 10mM EDTA, 5% glycerol, and 10mM DTT.\n\nStorage and Stability: \nLyophilized powder may be stored at -70 degrees C. Stable for 12 months at -70 degrees C. Reconstitute with sterile PBS, 15% glycerol. Aliquot to avoid repeated freezing and thawing. Store at -70 degrees C. Reconstituted product is stable for 6 months at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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