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Caspase- (Pro-form) (caspase 6, apoptosis-related cysteine peptidase, Apoptotic protease Mch-2, CASP-6, MCH2)

Caspase- (Pro-form) (caspase 6, apoptosis-related cysteine peptidase, Apoptotic protease Mch-2, CASP-6, MCH2)

Cat no: C2087-27T6

Supplier: United States Biological
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Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-6 is a major apoptotic executioner (1,2). It exists as a pro-domain protein and is cleaved by caspase-3 into two active subunits, an 18kD and a 10kD subunit (3). Caspase-6 cleaves among many different intracellular proteins, nuclear lamins. Lamins play a significant role in maintaining the integrity of the cell membrane, and their cleavage results in subsequent cellular breakdown and death (4). Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:10,000-1:20,000 Immunohistochemistry: 1:100-1:250 Immunocytochemistry: 1:100-1:250 Optimal dilutions to be determined by the researcher. Positive Control: MCF-7 cell lysates Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2087-27T6
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
P type: Mab
Isotype: IgG
Purity: Supernatant
References: 1. Srinivasula, S M., et al. (1996). J Biol Chem. 271:27099-27106. 2. Slee, E A., et al. (1999). J Cell Biol. 144:281-292. 3. Fernandes-Alnemri, T., et al. (1995). Cancer Res. 55:2737-2742. 4. Takahashi, A., et al. (1996). Proc Natl Acad Sci USA. 93:8395-8400.
Additional info: Recognizes human pro-Caspase-6.

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