Catalase BioAssay(TM) Kit

This Catalase BioAssay(TM) Kit detects high pico-unit of catalase in samples using either Absorbance- (570nm) or Fluorescence- (Ex/Em 535/587nm) based methods. Samples from mammalian species can be from cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, growth medium, food, etc. \n\nCatalase (EC 1.11.1.6) is a ubiquitous antioxidant enzyme that is present in nearly all living organisms. It functions to catalyze the decomposition of hydrogen peroxide (H202) to water and oxygen. The Catalase BioAssay(TM) Kit provides a highly sensitive, simple, direct and HTS-ready assay for measuring Catalase activity in biological samples. In the assay, catalase first reacts with H2O2 to produce water and oxygen, the unconverted H2O2 reacts with Oxidation/Reduction probe to produce a product, which can be measured at 570nm (Colorimetric method) or at Ex/Em=535/587nm (fluorometric method). Catalase activity is inversely proportional to the signal. The kit detects high pico-units of catalase in samples.\n\nKit Components:\nC2090-10A: Catalase *Assay Buffer, 1x25ml\nC2090-10B: Oxidation/Reduction Probe (in DMSO), 1x200ul\nC2090-10D: HRP, 1x1 vial\nC2090-10E: Hydrogen Peroxide (H2O2, 3%; 0.88M), 1x25ul\nC2090-10F: Stop Solution, 1x1ml\nC2090-10G: Catalase Positive Control, 1x1 vial\n\nStorage and Stability:\nStore the kit at -20 degrees C. Protect from light. Allow *C2090-10A: Catalase Assay Buffer to warm to RT before use. After reconstitution, components are stable for 2 weeks at 4 degrees C or for 1 month at -20 degrees C. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the caps.

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