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Catalase (CAT, MGC128112)

Catalase (CAT, MGC128112)

Cat no: C2090-02C

Supplier: United States Biological
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Catalase is a homotetrameric heme-containing enzyme present within the matrix of all peroxisomes. It carries out a dismutation reaction in which hydrogen peroxide is converted to water and oxygen. Human catalase has the last 4aa (-KANL) at the extreme C-terminus for peroxisome targeting. The monomer of human catalase is 61.3kD in molecular size. Catalase has been implicated as an important factor in inflammation, mutagenesis, prevention of apoptosis, and stimulation of a wide spectrum of tumors. Loss of catalase leads to the human genetic disease, acatalasemia, or Takahara's disease. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:2000 Immunoprecipitation: 2-5ug Optimal dilutions to be determined by the researcher. Positive Control: HeLa cells Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2090-02C
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in HEPES, 0.15M sodium chloride, 0.01% BSA, 0.03% sodium azide, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by Protein A affinity chromatography.
References: 1. Toiner, J. A. et al. (2000) J. Mol. Biol. 296(1):295-309.
Additional info: Recognizes human Catalase. Species Crossreactivity: mouse and rat.

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