The alpha-, beta- and gamma-catenins are cytoplasmic proteins mediating the interaction of Ca2+ -dependent transmembrane adhesion molecules (cadherins) with the cytoskeletal network. The direct interaction of beta-catenin with the cytoplasmic domain of cadherins plays a crucial role for cell-cell adhesion and signal transmission between neighboring cells. Recent studies indicate that beta-catenin may also play a role in tumorigenesis since it forms complexes with the tumor suppressor gene product APC. Beta-catenin directly interacts and constitutively activates transcription factors of the TCF/LEF gene family. Thus it is proposed that beta-catenin plays a dual role not only in the maintenance and regulation of cell-cell interactions but also in the regulation of gene activity.
Applications:
Suitable for use in ELISA, Western Blot, and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 0.5ug/ml
Immunocytochemistry: 1-10ug/ml
ELISA: 0.05ug/ml
Optimal dilutions to be determined by the researcher.
Positive Control (supplied):
Cell lysate of 2x10e6 LiCl treated SW480 cells. Supplied as a lyophilized powder. Reconstitute with 200ul sterile, ddH2O.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C for short-term only.Stable for 12 months at -20 degrees C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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