Beta-catenin is one of the key downstream effectors in the Wnt signaling pathway (1). It has been implicated in two major biological processes of vertebrates: early embryonic development (2) and tumorigenesis (3). GSK-3beta or other kinases may destabilize beta-catenin by phosphorylating it at serines 33, 37, 45 and/or threonine 41 (4). Stabilizing mutations of these phosphorylation sites in beta-catenin have been found in many tumor cell lines (5). Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser33, Ser37 and Thr41 of human beta-catenin. Corresponding antibody, C2096-61, Phospho-beta-Catenin (Ser33/37/Thr41), detects endogenous levels of beta-catenin only when phosphorylated at Ser33, Ser37 or Thr41. It does not recognize beta-catenin phosphorylated at other sites. Recognizes human, mouse, monkey.
Applications:
Suitable for use in ELISA, and as a blocking ragent to evaluate the specificity of antibody reactivity in Western Blot. Other applications have not been tested.
Recommended Dilution:
Western Blot:, 10ul of C2096-61A and 10ul of C2096-61 to 10ml of antibody dilution buffer, and incubate at RT for 2 hours before allowing to react with the blot.
Optimal dilutions to be determined by the researcher..
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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