Beta-catenin is one of the key downstream effectors in the Wnt signaling pathway (1). It has been implicated in two major biological processes of vertebrates: early embryonic development (2) and tumorigenesis (3). GSK-3beta or other kinases may destabilize beta-catenin by phosphorylating it at serines 33, 37, 45 and/or threonine 41 (4). Stabilizing mutations of these phosphorylation sites in beta-catenin have been found in many tumor cell lines (5).
Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr41 and Ser45 of human beta-catenin.
Corresponding antibody, C2096-62, Phospho-beta-Catenin (Thr41/Ser45), detects endogenous levels of beta-catenin only when phosphorylated at Thr41 or Ser45. This antibody does not recognize beta-catenin phosphorylated at other sites. Recognizes human, mouse, monkey
Applications:
Suitable for use in ELISA, Western Blot or for antigen applications in immunological protocols. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C or colder. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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