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Cathepsin B

Cat no: C2097

Cathepsin B

Cathepsin B is a cysteine protease commonly overexpressed in metastisizing cancer cells. An intracellular (lysosomal) enzyme in peptidase family C1 (papain family), Cathepsin B will hydrolyze proteins with broad specificity for peptide bonds. Preferentially cleaves -Arg-Arg bonds in small molecule substrates (thus differing from cathepsin L). In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides.\n\nSpecific Activity: \n(same/more than) 10u/mg enzyme activity\n\nUnit Definition:\nOne unit is defined as the amount of enzyme that will hydrolyze 1.0umol Z-RR-pNA per min at 37 degrees C, pH 6.8 in the presence of DTT. Note: One unit defined by Z-RR-pNA equals ~20 units defined with Z-RR-betaNA.\n\nMajor Bands:\n25-27kD, 30-33kD\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2097

Size

50ug

Form

Supplied as a liquid in 50mM sodium acetate buffer, pH 5.0, 1mM EDTA.

Purity

(same/more than) 95% (SDS-PAGE)

References

1. Bond, J.S. & Barrett, A.J., Biochem. J. 189: 17-25 (1980). 2. Barrett, A.J. & Kirschke, H., Methods Enzymol. 80: 535-561 (1981). 3. Polg

Alternative Names

EC=3.4.22.1; Cysteine Endopeptidases

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Applications

ELISA

Reactivities

Hum

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Applications

IF

Hosts

Mouse

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Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info
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