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Cathepsin D (CatD, CLN10, CPSD, CTSD, Lysosomal aspartyl peptidase, MGC2311)

Cat no: C2097-24C

Cathepsin D (CatD, CLN10, CPSD, CTSD, Lysosomal aspartyl peptidase, MGC2311)

Cathepsin D is a ubituitiously expressed lysosomal aspartyl protease involved in the normal degradation of proteins (1). It is synthesized as an inactive 43kD preprocathepsin D that is cleaved and glycosylated to form a 46kD procathepsin D and then further cleaved to produce 28kD and 15kD subunits (heavy and light chains, respectively) (2). Cathepsin D may also be secreted into the cytosol during apoptosis and contribute to cleavage of substrates implicated in the apoptotic pathway (3). Numerous studies have suggested that cathepsin D plays a role in neuronal degradation and malignant transformation, particularily in breast cancer (4-9).\n\nApplications: \nSuitable for use in Flow Cytometry, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot 1:1000\nImmunohistochemistry (Paraffin) 1:50\nFlow Cytometry 1:25\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2097-24C

Size

100ul

Applications

ELISA, FC, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA and 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1) Faust, P.L. et al. (1985) Proc. Natl. Acad. Sci. USA 82, 4910

Additional Info

Recognizes endogenous levels of human preprocathepsin D, procathepsin D and the heavy chain subunit of mature cathepsin D.

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info
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