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Cathepsin D (CatD, CLN10, CPSD, CTSD, Lysosomal aspartyl peptidase, MGC2311 )

Cathepsin D (CatD, CLN10, CPSD, CTSD, Lysosomal aspartyl peptidase, MGC2311 )

Cat no: C2097-24F

Supplier: United States Biological
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Cathepsin D is an aspartyl protease expressed in all cells and has a role on the degradation of proteins in lysosomes. It is initially expressed as an inactive 52kD precursor and activated by proteolysis. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Band at 34kD and weaker band at 52kD Immunohistochemistry (Frozen sections and paraffin-embedded sections): 1:200 for paraffin sections Optimal dilutions to be determined by the researcher. Recommended Positive Controls: Human breast carcinoma or cell line extract. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2097-24F
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Immunohistochemistry, Western Blot
Size: 100ul
Form: Supplied as a liquid, 15mM sodium azide
P type: Mab
Isotype: IgG2a
Purity: Ascites
Alternative names: CatD, CLN10, CPSD, CTSD, Lysosomal aspartyl peptidase, MGC2311
References: 1. Igdoura SA et al. Differential expression of cathepsins B and D in testis and epididymis of adult rats. J Histochem Cytochem 43:545-57 (1995). 2. Westley B & Rochefort H A secreted glycoprotein induced by estrogen in human breast cancer cell lines. Cell 20:353-62 (1980).
Additional info: Recognizes human Cathepsin D. Does not react with bovine cathepsin D and B, or with human cathepsin types B, C, G or H.

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