Cathepsin L (Cathepsin L heavy chain, Cathepsin L light chain, CATL, CTSL, Major excreted protein, MEP)

Cathepsins are lysosomal proteases that play an important role in the intracellular degradation of exogenous and endogenous proteins, activation of enzyme precursors, and tumor invasion and metastasis. Normally located in lysosomes of almost all mammalian cells, they can be secreted from the cell under certain conditions. Cathepsin L is responsible for most of the intralysosomal breakdown of normal cells. Cathepsin L is secreted by numerous transformed cells in its inactive proform, and mRNA expression level seems to correlate with their metastatic potential.\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunohistochemistry and Functional Studies. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:200\nImmunohistochemistry: frozen sections\nFunctional Studies: Suppresses the growth of malignant myeloma cells.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: Whole cell extract from cultured mink lung cell line.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2097-70J

Size

100ul

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid, 15mM sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

1. Sivaparvathi M et al. Expression and immunohistochemical localization of cathepsin L during the progression of human gliomas. Clin Exp Metastasis 14:27-34 (1996). <PUBMED:8521613>\n2. Igdoura SA et al. Differential expression of cathepsins B and D in testis and epididymis of adult rats. J Histochem Cytochem 43:545-57 (1995). <PUBMED:7730593>\n3. Weber E et al. Hybridoma cells producing antibodies to cathepsin L have greatly reduced potential for tumour growth. J Cancer Res Clin Oncol 120:564-7 (1994). <PUBMED:8045924>\n4. Mignatti P & Rifkin DB Biology and biochemistry of proteinases in tumor invasion. Physiol Rev 73:161-95 (1993). <PUBMED:8419965>\n5. Heidtmann HH et al. Secretion of a latent, acid activatable cathepsin L precursor by human non-small cell lung cancer cell lines. Oncol Res 5:441-51 (1993). <PUBMED:8054705>\n5. Yamaguchi N et al. Characterization of a cathepsin L-like enzyme secreted from human pancreatic cancer cell line HPC-YP. Cancer Res 50:658-63 (1990). <PUBMED:2297706>\n6. Trabandt A et al. Expression of the collagenolytic and Ras-induced cysteine proteinase cathepsin L and proliferation-associated oncogenes in synovial cells of MRL/I mice and patients with rheumatoid arthritis. Matrix 10:349-61 (1990). <PUBMED:2084514>\n7. Bellelli A et al. Inhibition of tumor growth, invasion and metastasis in papain-immunized mice. Invasion Metastasis 10:142-69 (1990). <PUBMED:2139872>\n8. Yagel S et al. Suppression by cathepsin L inhibitors of the invasion of amnion membranes by murine cancer cells. Cancer Res 49:3553-7 (1989). <PUBMED:2731177>\n9. Denhardt DT et al. Cysteine proteinase cathepsin L expression correlates closely with the metastatic potential of H-ras-transformed murine fibroblasts. Oncogene 2:55-9 (1987). <PUBMED:3438085>\n10. Kirschke H and Barrett AJ Chemistry of lysosomal proteases in lysosomes: Their role in protein breakdown Academic Press :193-239 (1981).\n11. Kirschke H et al. Cathepsin L. A new proteinase from rat-liver lysosomes. Eur J Biochem 74:293-301 (1977). <PUBMED:15835>

Additional Info

Recognizes native and denatured forms of human cathepsin L (25kD) and procathepsin L (42kD). Does not react with human cathepsin types B, D, H and S, procathepsins B and D, and procathepsin H, rat cathepsin B, and mouse procathepsins B and D. Species Crossreactivity: mouse procathepsin L (weak), rat cathepsin L (strong), and mink cathepsin and procathepsin L.

Alternative Names

Cathepsin L heavy chain, Cathepsin L light chain, CATL, CTSL, Major excreted protein, MEP