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Caveolin 1 (CAV1, Caveolae protein, HGNC:1527, CAV, MSTP085, Cell Growth-inhibiting Protein 32, VIP21)

Caveolin 1 (CAV1, Caveolae protein, HGNC:1527, CAV, MSTP085, Cell Growth-inhibiting Protein 32, VIP21)

Cat no: C2097-91Q

Supplier: United States Biological
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Caveolae are specialized domains of the plasma membrane that are implicated in the sequestration of a variety of lipid and protein molecules. It has been suggested that these important cellular organelles have a pivotal role in such diverse biochemical processes as lipid metabolism, growth regulation, signal transduction, and apoptosis. Caveolin interacts with and regulates heterotrimeric G-proteins. Currently, there are three members of the caveolin multigene family which are known to encode 21-24kD integral membrane proteins that comprise the major structural component of the caveolar membrane in vivo. Caveolin-2 protein is abundantly expressed in fibroblasts and differentiated adipocytes, smooth and skeletal muscle, and endothelial cells. The expression of caveolin-1 is similar to that of caveolin-2 while caveolin-3 expression appears to be limited to muscle tissue types. Applications: Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:4000 Immunofluorescence: 1:200 Optimal dilutions to be determined by the researcher. Positive Control: A431 and 3T3 whole cell lysates Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2097-91Q
Reactivities: Human, Mouse, Rat
Hosts: Mouse
Applications: Immunofluorescence, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in tris-glycine, 150mM NaCl, 0.05% sodium azide.
P type: Mab
Isotype: IgG2b
Purity: Purified by Protein A affinity chromatography.
Alternative names: CAV1, Caveolae protein, HGNC:1527, CAV, MSTP085, Cell Growth-inhibiting Protein 32, VIP21
References: 1. Souto R.P., et al. Immunopurification and characterization of rat adipocyte caveolae suggest their dissocation from insulin signaling. J. Biol. Chem. 278(20):18321-18329, 2003.
Additional info: Recognizes caveolin alpha and beta proteins. Species Crossreactivity: rat, human and mouse. Other species have not been tested.

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