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CBFb (CBFB, Core Binding Factor beta)

Cat no: C7900-76C

CBFb (CBFB, Core Binding Factor beta)

CBFb is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family that master-regulates a host of proteins specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters (1).\n\nApplications:\nSuitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and Flow Cytometry. Other applications have not been tested. \n\nRecommended Dilutions: \nWestern Blot: 1:1000-1:10,000 \nImmunocytochemistry: 1:100-1:250\nImmunohistochemistry: 1:100-1:250\nFlow cytometry: 1:100-1:500\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

C7900-76C

Size

100ul

Applications

FC, ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.05% BSA, 0.01% sodium azide, 50% glycerol.

P Type

Mab

Purity

Supernatant

References

1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1;33:D54-8.

Additional Info

Recognizes human CBFb. Species crossreactivity: Mouse and rat..

Read more on Supplier website

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

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