CBL (Lake Casitas, California mouse B-lineage Lymphoma; also RING finger protein 55) is a 120 -125kD cytosolic member of the CBL family of proteins. It is widely expressed, being found in cells such as fibroblasts, adipocytes, osteoclasts, podocytes and mammary epithelium. CBL negatively regulates RTK signaling by acting as an E3 ubiquitin ligase. Following activation via phosphorylation, CBL will interact with multiple signaling molecules such as Src, ZAP-70, EGFR and SHP1. Human CBL is 906aa in length. It contains an N-terminal phosphoTyr-binding region that consists of one a-helix bundle, an EF hand segment, and an SH2 domain (aa47-342), a RING finger domain that interacts with E2 enzymes (aa381-423) a Pro-rich SH3 domain (aa477-688), and a Leu-zipper/Ubiquitin-associated region (aa86-892). There is one potential alternative start site at Met55. Over (aa775-906, human CBL shares 91% aa identity with mouse CBL.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 0.5ug/ml
Optimal dilutions to be determined by the researcher.
Hybridoma:
Myeloma cells with B cells from Balb/c mice.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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