CTCF (CCCTC-binding factor) is a 130-140kD member of the CTCF Zn-finger protein family. Although its predicted MW is 83kD, it runs anomalously slow in SDS-PAGE. CTCF is ubiquitously expressed, save for primary spermatocytes, and serves multiple functions. It regulates both gene activation and repression by binding to unmethylated DNA sequences. Structurally, it contains eleven Zn-finger motifs, some or all of which may be involved in DNA binding. This variation in number allows for different conformations and multiple interaction partners. Human CTCF is 727 aa in length. It contains two SUMOylation sites (Lys74 and Lys689) and eleven consecutive C2H2-type Zn-finger domains (aa266-577). There are multiple Ser/Thr phosphorylation sites. Two potential alternative start sites exist; one nine aa upstream of thestandard start site, and another at Met570. A third splice variant shows a 62aa substitution for (aa349-727). Over (aa1-154), human CTCF shares 99% aa identity with mouse CTCF.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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