CCL19/MIP-3 beta BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human CCL19/MIP-3 beta in cell culture supernates, serum and plasma (heparin, EDTA).\n\nChemokine (C-C motif) ligand 19пїЅ(CCL19) is a smallпїЅcytokineпїЅbelonging to the CCпїЅchemokineпїЅfamily that is also known asпїЅEBI1 ligand chemokineпїЅ(ELC) andпїЅmacrophage inflammatory protein-3-betaпїЅ(MIP-3-beta).пїЅ Using PCR of human-rodent hybrids, the CCL19 gene was map to human chromosome 9p13, and the CCL19 gene is not part of the CC chemokine gene cluster on chromosome 17. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs.\n\nAssay Range:\n15.6pg/ml-1000pg/ml\n\nSensitivity:\n< 10 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nQ6IBD6\n\nEntrez Gene ID:\n6363\n\nKit Components:\n96-well plate pre-coated with anti-CCL19/MIP-3 beta, Human\nLyophilized recombinant CCL19/MIP-3 beta, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143748

Size

96Tests

References

1. Rossi, D. L., Vicari, A. P., Franz-Bacon, K., McClanahan, T. K., Zlotnik, A. Identification through bioinformatics of two new macrophage proinflammatory human chemokines. J. Immun. 158: 1033-1036, 1997. Note: Erratum: J. Immun. 163: 1091 only, 1999\n2. Yoshida, R., Imai, T., Hieshima, K., Kusuda, J., Baba, M., Kitaura, M., Nishimura, M., Kakizaki, M., Nomiyama, H., Yoshie, O. Molecular cloning of a novel human CC chemokine EBI1-ligand chemokine that is a specific ligand for EBI1, CCR7. J. Biol. Chem. 272: 13803-13809, 1997. \n