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CCNB1 (Cyclin B1, G2/Mitotic-specific Cyclin-B1, CCNB)

Cat no: C8453-04E


Supplier: United States Biological
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The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C8453-04E
Hosts: Mouse
Applications: Immunohistochemistry, Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
Additional info: Recognizes hamster CCNB1. Species sequence homology: Canine, guinea pig, human, mouse, porcine, rabbit, rat, sheep.

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