Receptors for the C-C chemokine family include CCR-1, CCR-2A, CCR-3, CCR-4, CCR-5 and the Duffy blood group antigen. The C-C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation.
Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested.
Recommended Dilution:
ELISA: 1:50,000
Western Blot: 1:1000
Immunohistochemistry (Frozen, Paraffin): 1:300
Flow Cytometry: 1:20
Optimal dilutions to be determined by the researcher.
Positive Control:
Human peripheral blood leukocytes or T-cell line Jurkatt.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.