CD1 is primarily expressed in cortical thymocytes and dendritic cells in the dermis, afferent lymph, lymph node, spleen and thymus, and also on B lymphocytes and monocytes. This antibody immunoprecipitates antigens at 12kD and 46kD. It is unreactive with granulocytes, platelets and erythrocytes.
Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry (frozen), and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 1:25-1:100; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.