CD103 (PE)

CD103 is a member of the integrin series of adhesion molecules. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and ~20% of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs, appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis and periphery. In non-epithelial CD103+ T cells there is a bias toward expression on CD8+ cells. This antibody is reported to have signal-inducing activity in a redirected lysis assay and to co-stimulate IEL and CD8+ lymph node cells in conjunction with anti-TCR in vitro, which runs counter to the usual inhibition of CTL by anti-integrins.1 The property of costimulation is not unique to the mouse since the combination of anti-human alphaIEL plus anti-TCR will produce similar proliferation in human cells, with the exception that CD8+ cells are not stimulated.\n\nApplications:\nSuitable for use in Flow Cytometry. Other applications have not been tested. \n\nRecommended Dilutions: \nFlow Cytometry: Neat. Use 10ul to label 10e6 cells in 100ul.\nOptimal dilutions to be determined by the researcher. \n\nRecommended Negative Controls: \nI1904-79D: IgG1 Murine Negative Control (PE)\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C. Reconstitute by adding sterile buffer or ddH2O. Aliquot and store at 4 degrees C. DO NOT FREEZE! Reconstituted product is stable for 12 months. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2445-63D

Size

100Tests

Applications

FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with R. Phycoerythrin (RPE). Reconstitute with 1ml sterile ddH2O.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

1. Flenghi, L. et al. (1990) LF61: a new monoclonal antibody directed against a trimeric molecule (150kD, 125kD, 105kD) associated with hairy cell leukaemia. Br. J. Haem. 76: 451-459.2. Falini, B. et al. (1990) Br. J. Haem 76:460-468.

Additional Info

Recognizes the human CD103 cell surface antigen, a glycoprotein expressed by approximately 1% of peripheral blood lymphocytes, activated T lymphocytes and by hairy cell leukaemia cells. The antigen is also expressed by intraepithelial lymphocytes. It has recently been shown to be identical to the alphaE integrin.