logo
logo
CD104 (Integrin beta4)

Cat no: C2446-02A

CD104 (Integrin beta4)

Applications: \nSuitable for use in Flow Cytometry and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 10ug/ml. Use 80ul to label 5x10e5 cells.\nOptimal dilutions to be determined by the researcher.\n\nFACS Protocol:\n1. Five x10e5 cultured UM-SCC (Squamous Cell Carcinoma) cells were harvested by trypsinization.\n2. Five x10e5 cells per tube were washed and pre incubated ~ 5 minutes with 20ul of 250ug/ml of human IgG.\n3. Incubate 5 x10e5 cells on ice for 45 minutes with 80ul of C2446-02A at 10ug/ml.\n4. Cells were washed twice and incubated with IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo.\n5. Washed 3x, fixed and analyzed on a BD FACS Calibur. \n6.. Cells stained positive with a mean shift of 1.78 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

C2446-02A

Size

100ug

Applications

FC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS pH 7.5, 0.5mg/ml gentamicin sulfate, <1% Bovine Immunoglobulin.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a

References

1. K.A. Kimmel and T.E. Carey, Cancer Res (1986) 46: 3614-3623. 2. C. VanWaes, et al, Cancer Res (1991) 51: 2395-2402. 3. Leukocyte Typing V (1995) S.F. Schlossman, et al, (eds.), Oxford University Press, NY. p. 1655-1663, 1667-1668.

Additional Info

Recognizes the human CD104 molecule at ~ 220kD (non-reduced). Partially blocks binding to laminin.

Read more on Supplier website
Advertisehere
MtoZ Horizontal banner
MtoZ Biolabs

Latest promotion FROM:

MtoZ Biolabs
View promotion

Latest promotions

Spend less time on DNA cleanup so you can do more science. The MSB Spin PCRapace is the fastest way to purify your DNA from PCR, restriction digestion, and...

New brilliant antibodies, and new lower prices!For flow cytometry reagents in general, \"bright is better.\" The violet-excitable BD Horizon&trade; BV421 and...

As an incentive to qualify our BSA, we are offering a 20% discount when you purchase your first 100g, 500g or 1000g of any grade of Bovine Serum Albumin....

It is not every day that you are given something for nothing. We are giving away additional spectrophotometer software.Cecil Instruments have enhanced the...

We're so sure that you'll prefer Cayman Assay kits over your present brand that we're willing to give you a free assay kit to prove it!

10% Discount on 2 Rabbit Polyclonal Antibody Service. With over 20 years experience, SDIX has developed into the premier US custom antibody producer,...

For the past decade scientists have extensively used ATS secondary toxin conjugates to make their own targeted toxins for in vitro use.The ability to combine...

Did your supplier increase the price of Fetal Bovine Serum? Did they substitute the US Origin with USDA? Well say no more! Innovative Research is still...

Bulk Cytokines with Custom Vialing.20 - 50% off cytokines, growth factors, chemokines and more...For a limited time Cell Sciences is offering substantial...

Jenway&rsquo;s 73 series spectrophotometer range provides four models with a narrow spectral bandwidth of 5nm and an absorbance range of &ndash;0.3 to 2.5A,...

Are you planning to have a customised antibody made for your research?Since 2000, Everest has been producing a catalog containing thousands of affinity...