CD115 (c-fms, Fms, CSF-1R, M-CSFR) (Azide Free)

CD115, a cell membrane associated antigen which is also known as c-fms, Macrophage Colony Stimulating Factor Receptor (M-CSFR) and CSF-1R. Two glycoproteins are recognized, with Mr=130kD and 150kD, corresponding to the immature and mature forms of CD115, respectively. The immature form (gp130c-fms) and the mature form \n(gp150c-fms) differ in composition of N-linked oligosaccharide chains. \nCD115 is an integral membrane protein with a 512 amino acid CSF-1 binding portion, a 25 amino acid membrane spanning portion and a 435 amino acid cytoplasmic portion. The cytoplasmic portion contains a tyrosine protein kinase domain. \nThis monoclonal antibody inhibits CSF-1 binding and ligand-dependent proliferation. It also interferes with CSF-1 independent growth observed in cells expressing a mutant oncogenic form of CSF-1R. It does not induce internalization or turnover of CSF-1R.\n\nApplications: \nSuitable for use in Immunohistochemistry and Immunofluorescence. Also suitable for use in blocking studies after removal of sodium azide. Other applications have not been tested.\n\nRecommended Dilution:\nImmunohistochemistry (acetone fixed cryostat sections only): 1:25 with a 30-60 minute incubation at 4 degrees C is recommended. \nOptimal dilutions to be determined by the researcher.\n\nRecommended Positive Control: \nTonsil, normal monocytes and macrophages and their committed bone marrow progenitors. Lymphocytes and granulocytes are not suitable for this application. \n\nHybridoma: \nRat splenocytes fused with rat myeloma Y3-Ag1.2.3 cells.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2447-53X

Size

100ug

Applications

IF, IHC

Hosts

Rat

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA.

P Type

Mab

Purity

Purified from ascites by ammonium sulfate precipitation.

Isotype

IgG1,k

References

1. Ashmun, R.A., et al., Blood 73(3): 827-837 (1989). 2. Sherr, C.J., et al., Blood 73(7): 1786-1793 (1989). 3. Ashmun, R.A., et al., CD115 (CSF-1 receptor) Cluster Workshop Report. In Leukocyte Typing V, edited by S.F. Schlossman, L. Boumsell, W. Gilks, J.M. Harlan, T. Kishimoto, C. Morimoto, J. Ritz, S. Shaw, R. Silverstein, T. Springer, T.F. Tedder, and R.F. Todd. Boston, MA. Oxford University Press. pp. 988-989 (1995).

Additional Info

Recognizes human CD115. The epitope for this antibody is between aa349 and 512. Does not crossreact with v-fms-coded glycoproteins. Species Crossreactivity: Does not crossreact with mouse or feline.