CD117, also known as c-kit, a 155kD type I transmembrane protein with protein tyrosine kinase activity, which plays an important role in early haematopoiesis. Haematopoietic stem cells (HSC) of bone marrow have multilineage differentiation potential and an extensive capacity for self-renewal. The majority of adult bone marrow haematopoietic progenitor cells are CD117+ and have been used successfully in xenograft transplantation models for long term survival of grafts, without symptoms of graft-versus-host disease (GVHD). C2448-05J recognizes CD117 on a small subset of porcine bone marrow progenitor cells and therefore provides an alternative tool from the previously used c-kit ligand stem cell factor, for the isolation and enrichment of porcine stem cells.
Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Western Blot. Shown as suitable for use on cytospins (1). Other applications not tested.
Recommended Dilution:
Flow Cytometry: 1:10-1:20; 10ul labels 10e6 cells in 100ul
Immunohistochemistry: Frozen
Western Blot: Detects a band of ~155kD in enriched porcine bone marrow cell lysates under non-reducing conditions. Enrichment of 2B8/BM+BMC population required by depletion of CD3+, 6D10+ and CD163+ cells (1).
Immunoprecipitation: Enrichment of 2B8/BM+BMC population required by depletion of CD3+, 6D10+ and CD163+ cells (1).
Optimal dilutions to be determined by the researcher.
Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Recommended Negative Control:
I1904-78R: IgG1 Murine Negative Control
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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