Integrin alpha-M (ITAM, ITGAM, CD11b, MAC-1 alpha subunit, CR3 alpha chain) is the alpha subunit of ITAM/beta-2 complex (CD11b/CD18, Mac-1), a leukocyte adhesion heterodimeric glycoprotein. ITAM belongs to integrin alpha chain family and it is predominately presented in human myeloid cells, NK1 cells, monocytes, and granulocytes. ITAM/beta-2 complex is a receptor for fibrinogen (binding to P1 and P1 peptides of gamma chain), factor X, and ICAM1. ITAM/beta-2 is implicated in adhesive interactions of monocytes, macrophages, and granulocytes. It is also linked to mediation of complement-coated particle uptake.
Applications:
Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions:
Western Blot: 1:1000-1:10,000
Immunohistochemistry (Paraffin): 1:100-1:250
Immunocytochemistry: 1:100-1:250
Flow Cytometry: 1:20
Immunoprecipitation: 1:80
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
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