CD11c, Recombinant, Human (Monocyte/Macrophage, CD11 Antigen-like Family Member C, Complement Component 3 Receptor 4 Subunit, Integrin alphaX, ITGAX, ITAX, Leukocyte Adhesion Glycoprotein p150,95 alpha Chain, Leukocyte Adhesion Receptor p150,95, Leu M5, L

CD11c antigen is expressed mainly on monocytes and in low density on granulocytes and NK cells. High levels of CD11c are expressed on tissue macrophages. It is also found on lymphoid cell lines including hairy cell leukemia. CD11c (integrin aX subunit) combines with CD18 (integrin b2 subunit) to form the integrin protein 150,95 (aXb2, CD11c/CD18). CD11c/CD18 has been reported to play an important role in cytotoxic T cell killing, and in neutrophil and monocyte adhesion to endothelium. Although its ligands in these two cases have not been identified. CD11c/CD18 is not expressed on leukocytes of patients with leukocyte adhesion deficiency. Leukocyte adhesion deficiency results from defects in the CD18 gene, leading to diminished expression of all CD18-containing integrins. CD11c antibodies bind multiple ligands including the complement fragment iC3b, CD54 (ICAM-1), fibrinogen and bacterial lippolysaccharide.\n\nSource:\nChinese Hamster Ovary cell line, Cho-derived\n\nSDS-PAGE: 149 & 103kD, reducing conditions.\n\nBiological Activity:\nMeasured by the ability of immobilized protein to support adhesion of J45.01 human acute T cell lymphoblastic leukemia cells. When 5X10e4 cells are added to rhIntegrin alphaXbeta2 coated plates (10ug/ml, 100ul/well), ~ 50%-80% will adhere after 1 hour at 37 degrees C.\n\nEndotoxin: (same/less than)1EU/ug (LAL)\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2262-60

Size

50ug

Form

Supplied as a lyophilized powder from 0.2um filtered solution in PBS. Reconstitute with sterile 200ug/ml PBS.

Purity

(same/more than)95% (SDS

References

1. Corbi, A.L. et al. (1987) EMBO J. 6:4023. 2. Kishimoto, T. K. et al. (1987) Cell 48:681. 3. Hynes, R.O. (2002) Cell 110:673. 4. Arnaout, M.A. (1990) Blood 75:1037. 5. Postigo, A. A. et al. (1991) J. Exp. Med. 174:1313. 6. Beyer, M. et al. (2005) Respir. Res. 6:70. 7. Nicolaou, F. et al. (2003) Blood 101:4033. 8. Vorup