Home  >  Products  >  CD13 (Alanyl Aminopeptidase, Aminopeptidase M, Aminopeptidase N, AP-M, APN, AP-N, CD13, gp150, GP150, hAPN, LAP1, Microsomal Aminopeptidase, Myeloid Plasma Membrane Glycoprotein CD13, p150, P150, PEPN)
CD13 (Alanyl Aminopeptidase, Aminopeptidase M, Aminopeptidase N, AP-M, APN, AP-N, CD13, gp150, GP150, hAPN, LAP1, Microsomal Aminopeptidase, Myeloid Plasma Membrane Glycoprotein CD13, p150, P150, PEPN)

CD13 (Alanyl Aminopeptidase, Aminopeptidase M, Aminopeptidase N, AP-M, APN, AP-N, CD13, gp150, GP150, hAPN, LAP1, Microsomal Aminopeptidase, Myeloid Plasma Membrane Glycoprotein CD13, p150, P150, PEPN)

Cat no: C2264-02C

Supplier: United States Biological
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Human CD13 is a zinc-binding aminopeptidase-N enzyme expressed on the surface of myeloid cells. IL-4 will upregulate expression of CD13 which may play an anti-inflammatory role. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: Formalin-fixed paraffin sections Flow Cytometry: 10ug/ml labels 5x10e5 ficoll prepared human Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured ficoll prepared human peripheral blood mononuclear cells were washed and preincubated 5min with 20ul human IgG (to block non-specific binding) after which they were incubated 45 min on ice with 80ul of C2264-02C at 10ug/ml. 2. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. 3. A net 5% subpopulation of the cells stained positive with a mean shift of 1.53 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2264-02C
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.5. No preservatives added.
P type: Mab
Isotype: IgG2a,k
Purity: Purified by Protein A affinity chromatography.
References: 1. M.A. Horton, et al, (1985) Cancer Res 45:5663-5669. 2. R.A. Ashmun & A.T. Look (1990) Blood 75:462-471. 3. P.T.W. van Hal, et al, (1994) J Immunol 153: 2718-2728.
Additional info: Recognizes the cell surface of human CD13.

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