The CD13 antigen is present on many non-haemopoietic tissues and represents an integral single chain membrane glycoprotein of 150kD. Present on monocytes and weakly expressed on granulocytes, it is only present on a small proportion of normal bone marrow cells. These comprise a major portion of the normal granulocyte-macrophage progenitor population. CD13 is a type III glycoprotein, the extracellular region of which contains the catalytic unit of aminopeptidase-N which is a zinc binding metalloprotease.
Applications:
Suitable for use in Flow Cytometry, ELISA, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 1:10 10ul labels 10e6 cells in 100ul.
Immunohistochemistry: frozen
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.