CD13 (APN, Alanyl Aminopeptidase, ANPEP, Aminopeptidase N, Membrane Protein p161)

Aminopeptidase N (APN) is also known as Alanyl Aminopeptidase (ANPEP), CD13 antigen or membrane protein p161. The protein consists of a short cyplasmic tail, a transmembrane region, a Ser/Thr region, and a zinc metalloproteinase domain. Widely expressed in many cells, tissues, and species, aminopeptidase N cleaves the N-terminal amino acids from bioactive peptides, leading to their inactivation or degradation. It has putative involvement in several biological processes including antigen processing and presentation, cell adhesion, tumor invasion and metastasis and neurotransmitter degradation. The antigen recognized by the mocnoclonal antibody is present on various mouse macrophage and dendritic cell subpopulations as well as on microvillous epithelia. The antigen is expressed at increasing levels upon maturation of macrophages. Therefore the monoclonal antibody is a useful marker for the identification of aminopeptidase N positive macrophages and dendritic cells in mouse, the expression of the antigen arises after the monocytic stage of differentation: bone marrow cells and peripheral blood monocytes are negative, whereas mature macrophages express the antigen. A subpopulation of dendrocytes and their in vitro equivalents, the dendritic cells, are also positive. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 Immunohistochemistry: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.