The CD13 antigen is present on most cells of myeloid origin, including granulocytes and monocytes in normal peripheral blood. CD13 is not expressed on B-cells, T-cells, platelets or erythrocytes. Expression of this antigen is greater on monocytes than on granulocytes.
Applications:
Suitable for use in Flow Cytometry, Direct ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilutions:
Flow Cytometry: 25ug/ml. Use 10ul to label 1-2.5x10e5 cells in total reaction volume (same/less than)200ul. Tested for use using U937 cells.
Direct ELISA: 0.5-1.0ug/ml. The detection limit for recombinant human CD13 is ~0.3ng/well.
Western Blot: 0.1-0.2ug/ml. The detection limit for recombinant human CD13 is ~5ng/lane under non-reducing and reducing conditions.
Immunoprecipitation: Precipitates CD13 from conditioned media of transfected NSO cells.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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