Home  >  Products  >  CD134 (ACT35 Antigen, OX40, OX40L Receptor, TAX Transcriptionally-activated Glycoprotein 1 Receptor, TXGP1L, Tumor Necrosis Factor Receptor Superfamily Member 4, TNFRSF4)
CD134 (ACT35 Antigen, OX40, OX40L Receptor, TAX Transcriptionally-activated Glycoprotein 1 Receptor, TXGP1L, Tumor Necrosis Factor Receptor Superfamily Member 4, TNFRSF4)

CD134 (ACT35 Antigen, OX40, OX40L Receptor, TAX Transcriptionally-activated Glycoprotein 1 Receptor, TXGP1L, Tumor Necrosis Factor Receptor Superfamily Member 4, TNFRSF4)

Cat no: C2515-19X

Supplier: United States Biological
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Human CD134 (OX40) (ACT35) is an activation-associated antigen which is predominantly expressed on activated CD4 positive cells. CD134 antigen is a member of the tumor necrosis factor (TNF) receptor family of molecules and may be involved with regulating T cell-dependent B cell proliferation and differentiation. Applications: Suitable for use in ELISA, Immunohistochemistry and Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ug/ml Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 cultured human HPB-MLT cells were incubated 45 minutes on ice with 80ul of C2515-19X at 10ug/mL. 2. Cells were washed twice and incubated with secondary reagent Goat anti-Mouse/FITC, after which they were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 1.10 log10 flourescent units when compared to a Mouse IgG1 negative control at a similar concentration. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2515-19X
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Flow Cytometry, Immunohistochemistry
Size: 100ug
Form: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride. No preservatives added.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography. >95% SDS-PAGE.
References: 1. Gramaglia I, et al. Immunol 161:6510-6517, 1998. 2. Humphreys IR, et al. JEM 198:1237-1242, 2003 General References: 1. U. Latza, et al, (1994) Eur J Immunol 24: 677-683, 2. E. Stuber, et al, (1995) Immunity 2: 507-521, 3. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 464-465, Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1157-1160.
Additional info: Recognizes the extracellular epitope of human CD134 at ~35kD. Does not block the interaction between CD134 (OX40) and CD252 (OX40L).

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