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CD134 Ligand, Blocking (CD134L, OX40L, CD252)

CD134 Ligand, Blocking (CD134L, OX40L, CD252)

Cat no: C2515-40

Supplier: United States Biological
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Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Functional Assays. Other applications not tested. Recommended Dilution: Flow Cytometry: 1ug/ml labels 5x10e5 cells. Functional Assays: Blocks binding of CD134L to CD134. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Rat TART-gp34 cells (5x10e5) expressing surface mOX40L vector or TART-1 parental cells, were incubated on ice for 30 min. in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1ug/ml of C2515-40. 2. After washing in FACS buffer, FITC-conjugated antibody to rat IgG was added. 3. Cells were incubated on ice for 30 min., washed and then analyzed by Flow Cytometry. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2515-40
Reactivities: Mouse
Hosts: Rat
Applications: Flow Cytometry, Immunoprecipitation, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 0.02% sodium azide, before the addition of glycerol to 40%.
P type: Mab
Isotype: IgG2c
Purity: Purified by immunoaffinity chromatography.
References: 1. Murata, K., et al., J. Exp. Med. 191: 365 (2000).
Additional info: Recognizes mouse CD134L. Does not crossreact with human CD134L.

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