Home  >  Products  >  CD134 Ligand (CD134L, CD252, Glycoprotein Gp34, GP34, OX40 Ligand, OX40L, OX-40L, TAX Transcriptionally-activated Glycoprotein 1, TXGP1, Tumor Necrosis Factor Ligand Superfamily Member 4, TNFSF4)
CD134 Ligand (CD134L, CD252, Glycoprotein Gp34, GP34, OX40 Ligand, OX40L, OX-40L, TAX Transcriptionally-activated Glycoprotein 1, TXGP1, Tumor Necrosis Factor Ligand Superfamily Member 4, TNFSF4)

CD134 Ligand (CD134L, CD252, Glycoprotein Gp34, GP34, OX40 Ligand, OX40L, OX-40L, TAX Transcriptionally-activated Glycoprotein 1, TXGP1, Tumor Necrosis Factor Ligand Superfamily Member 4, TNFSF4)

Cat no: C2515-40AX

Supplier: United States Biological
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Human CD134L (OX-40 Ligand) is a type II membrane protein with homology to TNF which is expressed on activated B cells and activated endothelial cells. CD134L binds to CD134 present on activated T cells, providing a costimulatory signal. This pathway seems to be more effective for costimulation of CD4+ helper T cells than for CD8+ effector T cell. Blockade of this interaction in mouse abrogated immunological effects in several models of immflamation and rejection. Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10mg/ml Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Cultured human umbilical cord vein endothelial cells were harvested by trypsinization, after which they were washed and incubated 45 minutes on ice with 80ul of C2515-40AX at 10mg/ml. 2. Cells were washed twice and incubated with secondary reagent Goat anti-Mouse IgG (FITC), after which they were washed three times, fixed and analyzed by FACS. 3. A net 14% subpopulation of the cells stained positive with a mean shift of 1.0 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2515-40AX
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Flow Cytometry
Size: 100ug
Form: Supplied as a liquid in PBS. No preservative added.
P type: Mab
Isotype: IgG1,k
Purity: Purified by Protein A affinity chromatography.
Alternative names: Tumor Necrosis Factor (Ligand) Superfamily, Member 4
References: 1. W. R. Godfrey, et al, (1994) J Exp Med 180: 757-763, 2. L.M. Higgins, et al, (1999) J Immunol 162: 486-493, 3. A.D. Weinberg, et al, (1999) J Immunol 162: 1818-1826, 4. V.Y. Taraban, et al, (2002) Eur J. Immunol. 32: 3617-3627, 5. V. Malmstrom, et al, (2001) J Immunol 166: 6972, 6. C. Nohara, et al, (2001) J Immunol 166: 2108-2115, 7. X. Yuan, et al, (2003) J Immunol 170: 2949-2955, 8. L. Tian, et al, (2002) Transplantation 74(1): 133-138.
Additional info: Recognizes human CD134 Ligand. Binds to CD134 Ligand on the cell surface of HUVEC in Flow Cytometry and blocks binding of recombinant CD134-muIg.

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