CD139 is a 228kD molecule expressed by germinal centre B cells, follicular dendritic cells and the follicular mantle. Smooth muscle of some blood vessels is also stained in some cases.
Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilutions:
Flow Cytometry: 1:10-1:50; 10ul labels 10e6 cells in 100ul
Immunohistochemistry (Frozen): The epitope recognized by this antibody is sensitive to formaldehyde fixation and tissue processing. Acetone fixation is recommended for frozen sections.
Optimal dilutions to be determined by the researcher.
Hybridoma:
Y3/Ag1.2.3 rat myeloma cells with spleen cells from immunized rats.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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