CD14 (B8, CD14, CIB8, CI-B8, Complex I-B8, NADH-Ubiquinone Oxidoreductase B8 Subunit)

Cat no: C2265-31J

Supplier: United States Biological

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Human CD14 is strongly expressed on myelomonocyte lineage cells including monocytes, macrophages, and a subset of granulocytes. CD14 is a high affinity receptor for complexes of lipopolysaccharide and lipopolysaccharide binding protein. A soluble form of CD14 also binds to LPS and LBP. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 ficoll prepared human peripheral blood mononuclear cells were washed and pre-incubated 5 min with 20microl human IgG at 250ug/ml (to block non specific binding) after which they were incubated 45 min on ice with 80ul of anti-CD14 antibody at 10ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A net 4.8% sub population of the cells stained positive with a mean shift of 1.6 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Catalogue number:	C2265-31J
Reactivities:	Human
Hosts:	Mouse
Applications:	Flow Cytometry
Size:	100ug
Form:	Supplied as a liquid in 50 mM sodium phosphate pH 7.5, 100 mM potassium chloride, 150mM sodium chloride. No preservative added.
P type:	Mab
Isotype:	IgG2a
Purity:	Purified by Protein A affinity chromatography.
References:	1. N. Hogg, et al, (1984) Immunology 53:753-767. 2. M.O. Labeta, et al, (1993) Eur J Immunol 23:2144- 2151, 3. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) "Reference Antibody", 4. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 778-788, 5. S.D. Wright, (1995) J Immunol 155:6-8.
Additional info:	Recognizes human CD14.

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CD14 (B8, CD14, CIB8, CI-B8, Complex I-B8, NADH-Ubiquinone Oxidoreductase B8 Subunit)

Cat no: C2265-31J

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