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CD14 (CD14 Antigen, Lipopolysaccharide Receptor, LPSR, Monocyte Differentiation Antigen 14, Myeloid Cell Specific Leucine Rich Glycoprotein)

Cat no: C2265-32P


Supplier: United States Biological
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The CD14 molecule is comprised of 365 amino acids and is anchored to the plasma membrane via a cleavable phosphoinositol linkage. CD14 is the LPS and LPS-binding protein receptor. Applications: Suitable for use in ELISA, Flow Cytometry, and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50-1:200; 5ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2265-32P
Reactivities: Human, Bovine, Canine, Feline, Goat, Non-Human Primate, Rabbit, Sheep
Hosts: Mouse
Applications: ELISA, Flow Cytometry, Western Blot
Size: 200ug
Form: Supplied as a liquid in PBS, 0.09% sodium azide.
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein G affinity chromatography.
Alternative names: CD14 antigen, Lipopolysaccharide receptor, LPSR, Monocyte Differentiation Antigen 14, Myeloid cell specific leucine rich glycoprotein
References: 1. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp. Anim. 49 (2): 97-110, 2. Soell, M et al. (1995) Activation of human monocytes by streptococcal rhamnose glucose polymers is mediated by CD14 antigen, and mannan binding protein inhibits TNF-alpha release. J. Immunol. 154: 851-860, 3. Gupta, V.K. et al. (1996) Identification of the sheep homologue of the monocyte cell surface molecule--CD14. Vet Immunol. Immunopathol. 51: 89-99, 4. Jacobsen, C.N. et al. (1993) Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species. Vet. Immunol. Immunopathol. 39: 461-466, 5. Sopp, P. et al. (1997) Cross-reactivity of monoclonal antibodies to defined human leucocyte differentiation antigens with bovine cells. Vet. Immunol. Immunopathol. 56: 11-25, 6. Arriens, M.A. et al. (1996) Cross reactivity of anti-human CD monoclonal antibodies with porcine leukocytes. Vet. Immunol. Immunopathol. 54: 113, 7. Werling, D et al. (1998) Analysis of the phenotype and phagocytic activity of monocytes/macrophages from cattle infected with the bovine leukaemia virus. Vet. Immunol. Immunopathol. 62:185-195, 8. Willett, B. et al. (2003) Expression of CXCR4 on feline peripheral blood mononuclear cells: effect of feline immunodeficiency virus infection. J. Virol. 77: 709 - 712, 9. Yang, H. et al. (2003) Antibody to CD14 like CXCR4-specific antibody 12G5 could inhibit CXCR4-dependent chemotaxis and HIV Env-mediated cell fusion. Immunol. Letters 88: 27-30.
Additional info: Recognizes the human CD14. Species crossreactivity: canine, goat, feline, rabbit, mink, bovine, porcine, sheep, cynomolgus monkey and llama.

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