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CD14 (Monocyte Differentiation Antigen CD14, Myeloid Cell-specific Leucine-rich Glycoprotein)

CD14 (Monocyte Differentiation Antigen CD14, Myeloid Cell-specific Leucine-rich Glycoprotein)

Cat no: C2265-55C1

Supplier: United States Biological
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CD14 is a 53-55kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed on macrophages, dendritic cells, Kupffer cells, hepatocytes, and granulocytes. As a high-affinity receptor for LPS-LBP (LPS-binding protein) complex, CD14, in association with Toll-like Receptor 4 (TLR4) or 2 (TLR2), is involved in the clearance of gram-negative pathogens. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: (same/less than)0.25ug/10e6 cells in 100ul volume Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2265-55C1
Reactivities: Mouse
Hosts: Rat
Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Size: 50ug
Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
P type: Mab
Isotype: IgG2a,k
Purity: Purified by immunoaffinity chromatography.
References: 1. Stocks S, et al. 1990. Biochem. J. 268:275. 2. Akashi S, et al. 2003. J. Exp. Med. 198:1035. 3. Matsuura K, et al. 1994. J. Exp. Med. 179:1671. 4. Liu S, et al. 1998. Infect. Immun. 66:5089.
Additional info: Recognizes mouse CD14.

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