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CD14, MONOCYTE, MACROPHAGE MARKER

CD14, MONOCYTE, MACROPHAGE MARKER

Cat no: C2265-51A

Supplier: United States Biological
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CD14 is a glycophosphatidylinositol-linked protein, which is part of the LPS receptor complex. CD14 binds lipopolysaccharide (LPS) and as such, acts as a pattern recognition receptor (PRR). CD14 has also been suggested to mediate phagocytosis of bacteria an apoptotic cells. CD14 is involved in the endotoxin mediated release of Tumor Necrosis Factor-alpha by monocytic cells. CD14 is present on most monocytic and macrophage like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is present at low density on a subpopulation granulocytes and activated or transformed B-cells. Applications: Suitable for use in Flow Cytometry, and for inhibition of biological activity of sCD14. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2265-51A
Reactivities: Human, Porcine
Hosts: Mouse
Applications: Flow Cytometry
Size: 100ug
Form: Supplied as a liquid in PBS, 0.1% BSA.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
References: 1. Tunheim, G et al; Human CD14 is an efficient target for recombinant immunoglobulin vaccine constructs that deliver T cell epitopes. J Leukoc Biol 2005, 77: 303 2. Wang, J et al; Peptidoglycan primes for LPS-induced release of proinflammatory cytokines in whole human blood. Shock 2001, 16: 178 3. Wang, J et al; Involvement of CD14 and toll-like receptors in activation of human monocytes by Aspergillus fumigatus hyphae. Infect Immun 2001, 69: 2402 4. Wang, J et al; Peptidoglycan and lipoteichoic acid from Staphylococcus aureus induce tumor necrosis factor alpha, interleukin 6 (IL-6), and IL-10 production in both T cells and monocytes in a human whole blood model. Infect Immun 2000, 68: 3965
Additional info: Recognizes human monocyte marker CD14, a 53kD glycoprotein. Recognizes both soluble and membrane CD14. Useful for inhibition of biological activity of sCD14, also inhibits LPS induced TNF production. Species crossreactivity: Porcine.

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