Thrombomodulin (THBD) is an endothelial-specific type I membrane receptor that inhibits coagulation and fibrinolysis. It functions primarily as a cell surface receptor and an essential cofactor for active thrombin, resulting in the activation of protein C, which in turn degrades clotting factors Va and VIIIa. Mutations in this gene can cause inherited thrombophilia. The aa sequence of human THBD is 99% and 76% identical to that of chimpanzee and mouse.
Applications:
Suitable for use in Flow Cytometry, ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilutions:
Flow Cytometry: 2.5ug/ml using THP-1 human acute monocytic leukemia cell line
Immunohistochemistry: 8-25ug/mlusing immersion fixed paraffin-embedded sections of human kidney
ELISA (Capture): 2-8ug/ml
ELISA (Detection): 0.5-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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