CD146, Recombinant, Human (Mucin 18, MUC18 Glycoprotein, Melanoma Associated Antigen A32, S-endo 1 Endothelial Associated Antigen, Melanoma Adhesion Molecule Mel-CM)

CD146 (aa433-647) belongs to the immunoglobulin supergene family with five immunoglobulin like domains (V-V-C2-C2-C2), a transmembrane region and a 63 residue cytoplasmic tail. The protein is a membrane glycoprotein that functions as a Ca2+ independent cell adhesion molecule involved in heterophilic cell to cell interactions. CD146 has a molecular size of 130kD in its reduced form (118kD unreduced), and N linked glycosylation accounts for fifty percent of the apparent molecular weight. In some cells the molecule carries a sulfate 3 glucuronyl moiety.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Inhibition assays. Other applications not tested.\n\nRecommended Dilutions: Optimal dilutions to be detrmined by the researcher.\n\nStorage and Stability:\nFor long-term storage, aliquot and stoe at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

C2548-78R

Size

2ug

Form

Supplied as a liquid in 50mM Tris-acetate, pH 7.5, 1mM EDTA, 20% glycerol.

Purity

Purified by proprietary chromatographic techniques. On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 47kD

References

1. Differential CD146 expression on circulating versus tissue endothelial cells in rectal cancer patients: implications for circulating endothelial and progenitor cells as biomarkers for antiangiogenic therapy. J Clin Oncol 2006 Mar 20;24(9):1449-53. 2. Knockdown of CD146 reduces the migration and proliferation of human endothelial cells. Cell Res 2006 Mar;16(3):313-8. 3. The first assessment of soluble CD146 in women with unexplained pregnancy loss. A new insight Thromb Haemost 2005 Dec;94(6):1280-4. 4. Presence of endothelial progenitor cells, distinct from mature endothelial cells, within human CD146+ blood cells. Thromb Haemost 2005 Dec;94(6):1270-9. 5. Increased expression of CD146, a new marker of the endothelial junction in active inflammatory bowel disease. Inflamm Bowel Dis 2006 Jan;12(1):16-21. 6. CD146 (Mel-CAM), an adhesion marker of endothelial cells, is a novel marker of lymphocyte subset activation in normal periphera l blood. Blood 2005 Oct 15;106(8):2923-4