Home  >  Products  >  CD15 (Alpha-(1,3)-fucosyltransferase, CD15, ELAM-1 ligand fucosyltransferase, ELFT, FCT3A, Fucosyltransferase 4, Fucosyltransferase IV, FucT-IV, Fuc-TIV, FUC-TIV, FUTIV, Galactoside 3-L-fucosyltransferase, LeX, SSEA-1)
CD15 (Alpha-(1,3)-fucosyltransferase, CD15, ELAM-1 ligand fucosyltransferase, ELFT, FCT3A, Fucosyltransferase 4, Fucosyltransferase IV, FucT-IV, Fuc-TIV, FUC-TIV, FUTIV, Galactoside 3-L-fucosyltransferase, LeX, SSEA-1)

CD15 (Alpha-(1,3)-fucosyltransferase, CD15, ELAM-1 ligand fucosyltransferase, ELFT, FCT3A, Fucosyltransferase 4, Fucosyltransferase IV, FucT-IV, Fuc-TIV, FUC-TIV, FUTIV, Galactoside 3-L-fucosyltransferase, LeX, SSEA-1)

Cat no: C2266-01T

Supplier: United States Biological
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Human CD15 is expressed by neutrophils, eosinophils and monocytes. Antibodies in this cluster recognize a common terminal pentasaccharide found on cell surface glycoproteins and glycolipids. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 cultured U-937 human tumor cells were incubated 45 min on ice with 80ul of anti-CD15 antibody at 5ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.47 log10 fluorescent units when compared to a Mouse IgM negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2266-01T
Reactivities: Human
Hosts: Mouse
Applications: ELISA
Size: 100ug
Form: Supplied as a liquid in 50 mM sodium phosphate pH 7.5, 500 mM potassium chloride, 150mM sodium chloride. No preservative added.
P type: Mab
Isotype: IgM
Purity: Purified by Protein A affinity chromatography.
References: 1. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 798-810, 2. M.A. Kerr & S.C. Stocks, (1992) Histochem J 24:811-826, 3. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 790-800.
Additional info: Recognizes human CD15.

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